Role of synectin in lymphatic development in zebrafish and frogs
Identifieur interne : 005636 ( Main/Exploration ); précédent : 005635; suivant : 005637Role of synectin in lymphatic development in zebrafish and frogs
Auteurs : Karlien Hermans [Belgique] ; Filip Claes [Belgique] ; Wouter Vandevelde [Belgique] ; Wei Zheng [Finlande] ; Ilse Geudens [Belgique] ; Fabrizio Orsenigo [Italie] ; Frederik De Smet [Belgique] ; Evisa Gjini [Pays-Bas] ; Kristof Anthonis [Belgique] ; Bin Ren [États-Unis] ; Dontcho Kerjaschki [Autriche] ; Monica Autiero [Belgique] ; Annelii Ny [Belgique] ; Michael Simons [États-Unis] ; Mieke Dewerchin [Belgique] ; Stefan Schulte-Merker [Pays-Bas] ; Elisabetta Dejana [Italie] ; Kari Alitalo [Finlande] ; Peter Carmeliet [Belgique]Source :
- Blood [ 0006-4971 ] ; 2010.
Abstract
The molecular basis of lymphangiogenesis remains incompletely characterized. Here, we document a novel role for the PDZ domain-containing scaffold protein synectin in lymphangiogenesis using genetic studies in zebrafish and tadpoles. In zebrafish, the thoracic duct arises from parachordal lymphangioblast cells, which in turn derive from secondary lymphangiogenic sprouts from the posterior cardinal vein. Morpholino knockdown of synectin in zebrafish impaired formation of the thoracic duct, due to selective defects in lymphangiogenic but not angiogenic sprouting. Synectin genetically interacted with Vegfr3 and neuropilin-2a in regulating lymphangiogenesis. Silencing of synectin in tadpoles caused lymphatic defects due to an underdevelopment and impaired migration of Prox-1+ lymphatic endothelial cells. Molecular analysis further revealed that synectin regulated Sox18-induced expression of Prox-1 and vascular endothelial growth factor C–induced migration of lymphatic endothelial cells
Url:
DOI: 10.1182/blood-2009-11-254557
PubMed: 20631376
PubMed Central: 3490102
Affiliations:
- Autriche, Belgique, Finlande, Italie, Pays-Bas, États-Unis
- Connecticut, Lombardie, Province du Brabant flamand, Région flamande, Utrecht (province), Uusimaa, Vienne (Autriche)
- Helsinki, Louvain, Milan, Utrecht, Vienne (Autriche)
- Katholieke Universiteit Leuven, Université d'Helsinki
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Le document en format XML
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uniqKey="Orsenigo F" first="Fabrizio" last="Orsenigo">Fabrizio Orsenigo</name><affiliation wicri:level="3"><nlm:aff id="aff4">IFOM, FIRC Institute of Molecular Oncology, Milan, Italy;</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>IFOM, FIRC Institute of Molecular Oncology, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="De Smet, Frederik" sort="De Smet, Frederik" uniqKey="De Smet F" first="Frederik" last="De Smet">Frederik De Smet</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research 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Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Autiero, Monica" sort="Autiero, Monica" uniqKey="Autiero M" first="Monica" last="Autiero">Monica Autiero</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author><author><name sortKey="Ny, Annelii" sort="Ny, Annelii" uniqKey="Ny A" first="Annelii" last="Ny">Annelii Ny</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author><author><name sortKey="Simons, Michael" sort="Simons, Michael" uniqKey="Simons M" first="Michael" last="Simons">Michael Simons</name><affiliation wicri:level="2"><nlm:aff id="aff6">Section of Cardiovascular Medicine, Yale University School of Medicine, New Haven, CT;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Connecticut</region></placeName><wicri:cityArea>Section of Cardiovascular Medicine, Yale University School of Medicine, New Haven</wicri:cityArea></affiliation></author><author><name sortKey="Dewerchin, Mieke" sort="Dewerchin, Mieke" uniqKey="Dewerchin M" first="Mieke" last="Dewerchin">Mieke Dewerchin</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author><author><name sortKey="Schulte Merker, Stefan" sort="Schulte Merker, Stefan" uniqKey="Schulte Merker S" first="Stefan" last="Schulte-Merker">Stefan Schulte-Merker</name><affiliation wicri:level="3"><nlm:aff id="aff5">Hubrecht Institute-KNAW and UMC, Utrecht, The Netherlands;</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Hubrecht Institute-KNAW and UMC, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Dejana, Elisabetta" sort="Dejana, Elisabetta" uniqKey="Dejana E" first="Elisabetta" last="Dejana">Elisabetta Dejana</name><affiliation wicri:level="3"><nlm:aff id="aff4">IFOM, FIRC Institute of Molecular Oncology, Milan, Italy;</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>IFOM, FIRC Institute of Molecular Oncology, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff8">Department of Biomolecular Sciences and Biotechnologies, University of Milan, Milan, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biomolecular Sciences and Biotechnologies, University of Milan, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name><affiliation wicri:level="4"><nlm:aff id="aff3">Molecular Cancer Biology Program, Biomedicum Helsinki, Haartman Institute, University of Helsinki, Helsinki, Finland;</nlm:aff><country xml:lang="fr">Finlande</country><wicri:regionArea>Molecular Cancer Biology Program, Biomedicum Helsinki, Haartman Institute, University of Helsinki, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName><orgName type="university">Université d'Helsinki</orgName></affiliation></author><author><name sortKey="Carmeliet, Peter" sort="Carmeliet, Peter" uniqKey="Carmeliet P" first="Peter" last="Carmeliet">Peter Carmeliet</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement 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type="wicri:Area/Ncbi/Curation">003C55</idno><idno type="wicri:Area/Ncbi/Checkpoint">003C55</idno><idno type="wicri:doubleKey">0006-4971:2010:Hermans K:role:of:synectin</idno><idno type="wicri:Area/Main/Merge">005684</idno><idno type="wicri:Area/Main/Curation">005636</idno><idno type="wicri:Area/Main/Exploration">005636</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Role of synectin in lymphatic development in zebrafish and frogs</title><author><name sortKey="Hermans, Karlien" sort="Hermans, Karlien" uniqKey="Hermans K" first="Karlien" last="Hermans">Karlien Hermans</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author><author><name sortKey="Claes, Filip" sort="Claes, Filip" uniqKey="Claes F" first="Filip" last="Claes">Filip Claes</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author><author><name sortKey="Vandevelde, Wouter" sort="Vandevelde, Wouter" uniqKey="Vandevelde W" first="Wouter" last="Vandevelde">Wouter Vandevelde</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author><author><name sortKey="Zheng, Wei" sort="Zheng, Wei" uniqKey="Zheng W" first="Wei" last="Zheng">Wei Zheng</name><affiliation wicri:level="4"><nlm:aff id="aff3">Molecular Cancer Biology Program, Biomedicum Helsinki, Haartman Institute, University of Helsinki, Helsinki, Finland;</nlm:aff><country xml:lang="fr">Finlande</country><wicri:regionArea>Molecular Cancer Biology Program, Biomedicum Helsinki, Haartman Institute, University of Helsinki, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation></author><author><name sortKey="Geudens, Ilse" sort="Geudens, Ilse" uniqKey="Geudens I" first="Ilse" last="Geudens">Ilse Geudens</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author><author><name sortKey="Orsenigo, Fabrizio" sort="Orsenigo, Fabrizio" uniqKey="Orsenigo F" first="Fabrizio" last="Orsenigo">Fabrizio Orsenigo</name><affiliation wicri:level="3"><nlm:aff id="aff4">IFOM, FIRC Institute of Molecular Oncology, Milan, Italy;</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>IFOM, FIRC Institute of Molecular Oncology, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="De Smet, Frederik" sort="De Smet, Frederik" uniqKey="De Smet F" first="Frederik" last="De Smet">Frederik De Smet</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author><author><name sortKey="Gjini, Evisa" sort="Gjini, Evisa" uniqKey="Gjini E" first="Evisa" last="Gjini">Evisa Gjini</name><affiliation wicri:level="3"><nlm:aff id="aff5">Hubrecht Institute-KNAW and UMC, Utrecht, The Netherlands;</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Hubrecht Institute-KNAW and UMC, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Anthonis, Kristof" sort="Anthonis, Kristof" uniqKey="Anthonis K" first="Kristof" last="Anthonis">Kristof Anthonis</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author><author><name sortKey="Ren, Bin" sort="Ren, Bin" uniqKey="Ren B" first="Bin" last="Ren">Bin Ren</name><affiliation wicri:level="2"><nlm:aff id="aff6">Section of Cardiovascular Medicine, Yale University School of Medicine, New Haven, CT;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Connecticut</region></placeName><wicri:cityArea>Section of Cardiovascular Medicine, Yale University School of Medicine, New Haven</wicri:cityArea></affiliation></author><author><name sortKey="Kerjaschki, Dontcho" sort="Kerjaschki, Dontcho" uniqKey="Kerjaschki D" first="Dontcho" last="Kerjaschki">Dontcho Kerjaschki</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="aff7">Institute of Clinical Pathology, Medical University of Vienna, Vienna, Austria</nlm:aff><country xml:lang="fr">Autriche</country><wicri:regionArea>Institute of Clinical Pathology, Medical University of Vienna, Vienna</wicri:regionArea><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Autiero, Monica" sort="Autiero, Monica" uniqKey="Autiero M" first="Monica" last="Autiero">Monica Autiero</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author><author><name sortKey="Ny, Annelii" sort="Ny, Annelii" uniqKey="Ny A" first="Annelii" last="Ny">Annelii Ny</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author><author><name sortKey="Simons, Michael" sort="Simons, Michael" uniqKey="Simons M" first="Michael" last="Simons">Michael Simons</name><affiliation wicri:level="2"><nlm:aff id="aff6">Section of Cardiovascular Medicine, Yale University School of Medicine, New Haven, CT;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Connecticut</region></placeName><wicri:cityArea>Section of Cardiovascular Medicine, Yale University School of Medicine, New Haven</wicri:cityArea></affiliation></author><author><name sortKey="Dewerchin, Mieke" sort="Dewerchin, Mieke" uniqKey="Dewerchin M" first="Mieke" last="Dewerchin">Mieke Dewerchin</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author><author><name sortKey="Schulte Merker, Stefan" sort="Schulte Merker, Stefan" uniqKey="Schulte Merker S" first="Stefan" last="Schulte-Merker">Stefan Schulte-Merker</name><affiliation wicri:level="3"><nlm:aff id="aff5">Hubrecht Institute-KNAW and UMC, Utrecht, The Netherlands;</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Hubrecht Institute-KNAW and UMC, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Dejana, Elisabetta" sort="Dejana, Elisabetta" uniqKey="Dejana E" first="Elisabetta" last="Dejana">Elisabetta Dejana</name><affiliation wicri:level="3"><nlm:aff id="aff4">IFOM, FIRC Institute of Molecular Oncology, Milan, Italy;</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>IFOM, FIRC Institute of Molecular Oncology, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff8">Department of Biomolecular Sciences and Biotechnologies, University of Milan, Milan, Italy</nlm:aff><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biomolecular Sciences and Biotechnologies, University of Milan, Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name><affiliation wicri:level="4"><nlm:aff id="aff3">Molecular Cancer Biology Program, Biomedicum Helsinki, Haartman Institute, University of Helsinki, Helsinki, Finland;</nlm:aff><country xml:lang="fr">Finlande</country><wicri:regionArea>Molecular Cancer Biology Program, Biomedicum Helsinki, Haartman Institute, University of Helsinki, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName><orgName type="university">Université d'Helsinki</orgName></affiliation></author><author><name sortKey="Carmeliet, Peter" sort="Carmeliet, Peter" uniqKey="Carmeliet P" first="Peter" last="Carmeliet">Peter Carmeliet</name><affiliation wicri:level="1"><nlm:aff id="aff1">Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Vlaams Iustituut voor Biotechnologie (VIB), Leuven</wicri:regionArea><wicri:noRegion>Leuven</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2">Vesalius Research Center, Katholieke Universiteit Leuven, Leuven, Belgium;</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Vesalius Research Center, Katholieke Universiteit Leuven, Leuven</wicri:regionArea><orgName type="university">Katholieke Universiteit Leuven</orgName><placeName><settlement type="city">Louvain</settlement><region>Région flamande</region><region type="district" nuts="2">Province du Brabant flamand</region></placeName></affiliation></author></analytic><series><title level="j">Blood</title><idno type="ISSN">0006-4971</idno><idno type="eISSN">1528-0020</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The molecular basis of lymphangiogenesis remains incompletely characterized. Here, we document a novel role for the PDZ domain-containing scaffold protein synectin in lymphangiogenesis using genetic studies in zebrafish and tadpoles. In zebrafish, the thoracic duct arises from parachordal lymphangioblast cells, which in turn derive from secondary lymphangiogenic sprouts from the posterior cardinal vein. Morpholino knockdown of synectin in zebrafish impaired formation of the thoracic duct, due to selective defects in lymphangiogenic but not angiogenic sprouting. Synectin genetically interacted with Vegfr3 and neuropilin-2a in regulating lymphangiogenesis. Silencing of synectin in tadpoles caused lymphatic defects due to an underdevelopment and impaired migration of Prox-1<sup>+</sup> lymphatic endothelial cells. Molecular analysis further revealed that synectin regulated Sox18-induced expression of Prox-1 and vascular endothelial growth factor C–induced migration of lymphatic endothelial cells <italic>in vitro</italic>. These findings reveal a novel role for synectin in lymphatic development.</p></div></front></TEI><affiliations><list><country><li>Autriche</li><li>Belgique</li><li>Finlande</li><li>Italie</li><li>Pays-Bas</li><li>États-Unis</li></country><region><li>Connecticut</li><li>Lombardie</li><li>Province du Brabant flamand</li><li>Région flamande</li><li>Utrecht (province)</li><li>Uusimaa</li><li>Vienne (Autriche)</li></region><settlement><li>Helsinki</li><li>Louvain</li><li>Milan</li><li>Utrecht</li><li>Vienne (Autriche)</li></settlement><orgName><li>Katholieke Universiteit Leuven</li><li>Université d'Helsinki</li></orgName></list><tree><country name="Belgique"><noRegion><name sortKey="Hermans, Karlien" sort="Hermans, Karlien" uniqKey="Hermans K" first="Karlien" last="Hermans">Karlien Hermans</name></noRegion><name sortKey="Anthonis, Kristof" sort="Anthonis, Kristof" uniqKey="Anthonis K" first="Kristof" last="Anthonis">Kristof Anthonis</name><name sortKey="Anthonis, Kristof" sort="Anthonis, Kristof" uniqKey="Anthonis K" first="Kristof" last="Anthonis">Kristof Anthonis</name><name sortKey="Autiero, Monica" sort="Autiero, Monica" uniqKey="Autiero M" first="Monica" last="Autiero">Monica Autiero</name><name sortKey="Autiero, Monica" sort="Autiero, Monica" uniqKey="Autiero M" first="Monica" last="Autiero">Monica Autiero</name><name sortKey="Carmeliet, Peter" sort="Carmeliet, Peter" uniqKey="Carmeliet P" first="Peter" last="Carmeliet">Peter Carmeliet</name><name sortKey="Carmeliet, Peter" sort="Carmeliet, Peter" uniqKey="Carmeliet P" first="Peter" last="Carmeliet">Peter Carmeliet</name><name sortKey="Claes, Filip" sort="Claes, Filip" uniqKey="Claes F" first="Filip" last="Claes">Filip Claes</name><name sortKey="Claes, Filip" sort="Claes, Filip" uniqKey="Claes F" first="Filip" last="Claes">Filip Claes</name><name sortKey="De Smet, Frederik" sort="De Smet, Frederik" uniqKey="De Smet F" first="Frederik" last="De Smet">Frederik De Smet</name><name sortKey="De Smet, Frederik" sort="De Smet, Frederik" uniqKey="De Smet F" first="Frederik" last="De Smet">Frederik De Smet</name><name sortKey="Dewerchin, Mieke" sort="Dewerchin, Mieke" uniqKey="Dewerchin M" first="Mieke" last="Dewerchin">Mieke Dewerchin</name><name sortKey="Dewerchin, Mieke" sort="Dewerchin, Mieke" uniqKey="Dewerchin M" first="Mieke" last="Dewerchin">Mieke Dewerchin</name><name sortKey="Geudens, Ilse" sort="Geudens, Ilse" uniqKey="Geudens I" first="Ilse" last="Geudens">Ilse Geudens</name><name sortKey="Geudens, Ilse" sort="Geudens, Ilse" uniqKey="Geudens I" first="Ilse" last="Geudens">Ilse Geudens</name><name sortKey="Hermans, Karlien" sort="Hermans, Karlien" uniqKey="Hermans K" first="Karlien" last="Hermans">Karlien Hermans</name><name sortKey="Ny, Annelii" sort="Ny, Annelii" uniqKey="Ny A" first="Annelii" last="Ny">Annelii Ny</name><name sortKey="Ny, Annelii" sort="Ny, Annelii" uniqKey="Ny A" first="Annelii" last="Ny">Annelii Ny</name><name sortKey="Vandevelde, Wouter" sort="Vandevelde, Wouter" uniqKey="Vandevelde W" first="Wouter" last="Vandevelde">Wouter Vandevelde</name><name sortKey="Vandevelde, Wouter" sort="Vandevelde, Wouter" uniqKey="Vandevelde W" first="Wouter" last="Vandevelde">Wouter Vandevelde</name></country><country name="Finlande"><region name="Uusimaa"><name sortKey="Zheng, Wei" sort="Zheng, Wei" uniqKey="Zheng W" first="Wei" last="Zheng">Wei Zheng</name></region><name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name></country><country name="Italie"><region name="Lombardie"><name sortKey="Orsenigo, Fabrizio" sort="Orsenigo, Fabrizio" uniqKey="Orsenigo F" first="Fabrizio" last="Orsenigo">Fabrizio Orsenigo</name></region><name sortKey="Dejana, Elisabetta" sort="Dejana, Elisabetta" uniqKey="Dejana E" first="Elisabetta" last="Dejana">Elisabetta Dejana</name><name sortKey="Dejana, Elisabetta" sort="Dejana, Elisabetta" uniqKey="Dejana E" first="Elisabetta" last="Dejana">Elisabetta Dejana</name></country><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="Gjini, Evisa" sort="Gjini, Evisa" uniqKey="Gjini E" first="Evisa" last="Gjini">Evisa Gjini</name></region><name sortKey="Schulte Merker, Stefan" sort="Schulte Merker, Stefan" uniqKey="Schulte Merker S" first="Stefan" last="Schulte-Merker">Stefan Schulte-Merker</name></country><country name="États-Unis"><region name="Connecticut"><name sortKey="Ren, Bin" sort="Ren, Bin" uniqKey="Ren B" first="Bin" last="Ren">Bin Ren</name></region><name sortKey="Simons, Michael" sort="Simons, Michael" uniqKey="Simons M" first="Michael" last="Simons">Michael Simons</name></country><country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Kerjaschki, Dontcho" sort="Kerjaschki, Dontcho" uniqKey="Kerjaschki D" first="Dontcho" last="Kerjaschki">Dontcho Kerjaschki</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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